Large size moth-eye nanostructures were successfully prepared on the surface of the polymer films by roll-to-plate (RTP) UV nano-imprint lithography. By atomic force microscopy (AFM), it is shown that the moth-eye nanostructure on the surface of polymer is more regular, with milky bulges arranged in arrays. The replication rate of the wave height to the moth-eye nanostructure could reach 95.2%. The microstructure from the surface is filled completely and effectively. Compared with the single coating (AR-coating), the reflectance test results of the moth-eye nanostructure are reduced to 3% in the visible light range (380～760 nm). The average reflectance of visible light at 110° is 7%。