文章摘要
聚L-谷氨酸/壳聚糖纳米凝胶的制备与表征
Preparation and Characterization of Poly(L-glutamic acid)/Chitosan Nanogels
  
DOI:10.16865/j.cnki.1000-7555.2018.02.028
中文关键词: 聚L-谷氨酸  纳米凝胶  药物释放
英文关键词: poly(L-glutamic acid)  nanogels  drug release
基金项目:国家自然科学基金资助项目(51473090,51373094);上海市自然科学基金资助项目(14ZR1414600);上海市科学技术委员会基础研究项目(15JC1490400)
作者单位
颜世峰,李 星,简宇航,吴念琦,滕畅畅,朱雨雯,尹静波 上海大学 材料科学与工程学院 高分子材料系,上海 200444 
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中文摘要:
      以纳米SiO2 为模板,在其表面接枝聚L-谷氨酸(PLGA),以壳聚糖(CS)为大分子交联剂,构建了PLGA/CS化学交联中空纳米凝胶,研究了纳米SiO2 粒子表面接枝,纳米凝胶制备、微观形貌以及pH 响应行为。以水溶性盐酸米托蒽醌(MTX)为模型药物,研究了PLGA/CS纳米凝胶的载药与释药性能。结果表明,PLGA 在SiO2 表面成功接枝;PLGA/CS纳米凝胶呈球形,平均粒径为365nm。冻干后纳米凝胶尺寸收缩至100nm 左右;PLGA/CS纳米凝胶具有pH响应性,随pH 的提高,PLGA/CS纳米凝胶粒径先减小后增大;PLGA/CS纳米凝胶对MTX有良好的负载能力,最高载药量达41.4%。载MTX的PLGA/CS纳米凝胶在起始阶段存在一定程度突释,随后释药速度明显变缓,直到7d后达到释放平衡,药物缓释效果良好。
英文摘要:
      The SiO2 nanospheres with good monodispersity were successfully prepared by Stber method. And poly (L-glutamic acid) (PLGA) was grafted to the surface. PLGA/CS hollow nanogels were obtained using chitosan (CS) as macromolecular crosslinking agent. Then, the PLGA/CS nanogels were obtained after SiO2 core removal. The preparation, micromorphology, pH-responsiveness, drug loading capacity and release of PLGA/CS hollow nanogels were characterized by nuclear magnetic resonance (NMR) spectroscopy, infrared (IR) spectroscopy, light scattering, and transmission electron microscopy (TEM). Taking water-soluble antineoplastic agent mitoxantrone (MTX) as model drug, the drug loading and release performance were characterized by confocal laser scanning microscopy (CLSM) and ultraviolet visible spectrophotometer (UV-vis). The results show that PLGA is successfully grafted onto the surface of SiO2 nanospheres. The PLGA/CS nanogels are spherical in shape and uniform in particle size. The average diameter is about 365 nm. After freeze-drying, the size of PLGA/CS nanogels is shrinked to ca. 100 nm. The PLGA/CS nanogels show pH-dependent behavior. With the increase of pH, the particle size decreases first and then increases.PLGA/CS nanogels display high loading capacity of MTX with the maximum value of 41.4%, which is mainly attributed to the electrostatic interaction between MTX and -COO-. The PLGA/CS nanogels show sustained release behavior. The MTX-loaded PLGA/CS nanogels show a certain degree of burst release at the initial stage, and then the release rate is slowed down and finally reaches equilibrium after 7 d.
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