Abstract:Calcium alginate hydrogel is a kind of well biocompatible and biodegradable biomedical polymer material. However, hydrogel with physical structure cannot be obtained by traditional preparation method. This paper proposed an electrodeposition method of fabricating shape-controlled calcium alginate-poly-L-lysine (PLL) hydrogel microcapsules. The micro-patterned electrodes, which were produced by coating a patterned photoresist layer onto FTO glass slide based on photolithography technique, were used for making different shapes of alginate hydrogel structures. Then, the structures would be detached from the electrode surface and treated with given reagent to be transformed into 3D microcapsules while maintaining the ring and hexagon shapes. Finally, the yeast cells within microcapsules can maintain certain bioactivity after 24h’ cultivation. The recommended method can lead to fabricating cell-laden scaffold for tissue engineering. It will be instructive in the field of cells assembly, bio-printing and drug discovery.