The multiple amphiphilic comb polymers were prepared by radical polymerization. Structural properties of copolymers were tested by ¹H-NMR, FT-IR, gel-permeation chromatography (GPC) and other instruments. When the mass of the hydrophilic group is less than 60% of the total amount of monomer, the molecular weight of the polymer increases with increase of the content of the hydrophilic group, and the molecular weight distribution is uniform. Detect the variation law of the polymer solution viscosity by Ubbelohde viscometer，the results show that the content of the hydrophilic groups of the polymer could not affect the concentration of the polymer in aqueous solution. The critical micelle concentration of this series of polymers is 0.3 g/L. The results of dynamic light scattering (DLS) test show that the size of the micelles increases with increase of the concentration of the polymer solution, the more hydrophilic groups, the larger the size of the micelles, the more uneven distribution of micelles. TEM photos show that the polymeric micelles are spherical micelle with core-shell structure.