In this study, polyester diol (PEA2000, PBA2000, PHA2000) with a molecular weight of 2000 was used as monomer. Various molecular weights of polyester polyurethane acrylate macromolecular crosslinkers were obtained by adjusting the mole ratio of isocyanate to hydroxyl (n(-NCO)∶n(-OH)), then mixed with functional monomers acrylic acid （AA）and 2-acrylamide-2-methylpropane sulfonic acid (AMPS), small molecule crosslinker N,N-methylenebisacrylamide (MBA) and KCl in the a mixture of glycerol and water. The solution was prepared and UV cured for making pressure sensitive adhesive(PSA). The composition and structure of the macromolecular cross-linker were characterized. The results show that by adjusting the mole ratio of isocyanate to hydroxyl group, polyurethane macromolecular cross-linkers of various molecular weight can be obtained; then the performance of pressure sensitive adhesives was characterized. PSAs shows the best mechanical property when PUA-PBA2000 (the mole ratio of n(-NCO)n(-OH) is 1.4) is used as macromolecular crosslinker. PSAs has a good conductivity and hydrophilicity, indicating a very good application prospect in medical electrodes.