The natural product chitosan modified with maleic anhydride was introduced into the thermoresponsive hydrogels, poly N-isopropyl acrylamide (PNIPAAm). Effect of N-malay acylation chitosan (N-MACH) on the thermoresponsive, swelling, compressive strength, and degradation of the hydrogels was investigated. The results suggest that when the content of N-MACH is 30%, the synthesized hydrogels not only have better temperature sensitivity and swelling performance, but also have a compression strength 311.2% higher than that of PNIPAAm hydrogels only crosslinked with BIS. In enzymatic degradation, the mass loss of the hydrogel with 30% N-MACH could be added up to 60.8% in the solution of cellulase (8 U/mL) at 40 ℃ and pH 5.0, which furtherly provide the foundation for the application of chitosan crosslinked hydrogels in the biological field.