Using isomannitol (IMT), hexamethylene diisocyanate (HDI), 1,4-butanediol, and glycerol (GI) as raw materials, the bio-based Vitrimer material IMT-GI was designed and synthesized. Its structure was characterized by Fourier transform infrared spectroscopy (FT-IR) and X-ray diffraction (XRD). The thermal stability, glass transition temperature, mechanical properties and storage modulus of IMT-GI material were investigated by thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), stress-strain curve and dynamic mechanical analysis (DMA). The results show that: IMT-COO has good thermal stability and high mechanical strength, the thermal decomposition temperature reaches 256 ℃, the stress is 1.24 MPa, and the strain is 140 %. The storage modulus of IMT-COO in the DMA test is up to 2671MPa. Through the self-healing test of IMT-COO, it shows that there is no obvious decrease in various properties before and after self-healing.