PEG对角蛋白游离巯基的保护作用及其对材料性能的影响
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江南大学 生态纺织教育部重点实验室,江苏 无锡 214122

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国家自然科学基金面上项目(31771039)


Protective Effect of PEG on Free Thiol of Keratin and Its Effect on Material Properties
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    摘要:

    游离巯基很容易连接形成二硫键,对角蛋白溶液的稳定性及再生蛋白韧性都会产生不良影响,因此对巯基进行适当保护有助于提升角蛋白的性能。为此,本文选择聚乙二醇(PEG)、聚乙烯醇(PVA)以及十二烷基硫酸钠(SDS)三种物质,分析比较了它们对巯基的保护效果。之后以保护效果最好的PEG为例,分析了其分子量和浓度对巯基保护效果的影响,同时采用动态光散射、荧光光谱以及拉曼光谱进行了胶束粒径、疏水氨基酸暴露程度和角蛋白二级构象等测试表征。结果显示:PEG可以通过疏水作用力和氢键等方式与角蛋白分子间形成高分子复合物,PEG分子量越大,浓度越高,所形成的复合物粒径就越大,保护效果也越好;而且复合物使得角蛋白分子舒展,减弱了巯基无序交联形成二硫键比例,在固化后的角蛋白混合膜中,二级结构变得规整有序,膜的柔韧性和透光性均显著增加。

    Abstract:

    Free thiol groups are easily connected to form disulfide bonds, which will adversely affect the stability of keratin solution and the properties of regenerated keratin. Therefore, proper protection of thiol groups can help improve the performance of keratin. In this paper, polyethylene glycol (PEG), polyvinyl alcohol (PVA) and sodium dodecyl sulfate (SDS) was employed as the main compounds and their protective effects on thiol groups was detailed studied. Then, PEG with the best protection effect was chosen as the main study object. The influence of PEG molecular weight and concentration on the protection effect of sulfhydryl groups was further analyzed. The micelle particle size, hydrophobic amino acid exposure ratio, and the secondary conformation were also characterized by using DLS, Fluorescence spectroscopy, and Raman spectroscopy. The results showed that PEG can form polymer complexes with keratin molecules through hydrophobic interaction, hydrogen bonding. Moreover, the Ker/PEG complex made the keratin molecules stretch and weaken the disordered cross-linking of thiol groups. Compared with the keratin film, the secondary structure of PEG/Ker became much more regular, and the flexibility and light transmittance of the membrane was also significantly increased.

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  • 收稿日期:2022-06-22
  • 录用日期:2023-02-07
  • 网络出版日期:2022-12-09
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