Temperature responsive non-traditional fluorescent polymers with amide bond in the backbones were prepared via phosphazene base (t-BuP₂) catalyzed oxa-Michael addition polymerization of trimethylolpropane (TMP) and N,N-methylenebisacrylamide (MBA) at room temperature. The structure and molecular weight of polymers were characterized by flourier transform infrared spectroscopy (FT-IR), nuclear magnetic resonance spectroscopy (NMR) and gel permeation chromatography (GPC). The fluorescence properties and temperature responsive behavior of polymers were explored by ultraviolet spectrophotometer (UV-vis) and fluorescence analysis. The results show that the polymer with amide bond in the backbones shows the aggregation-induced emission (AIE) and excitation wavelength-dependent characteristics. More importantly, the polymer containing amide bond in the backbones has a temperature responsiveness and lower critical solution temperature (LCST) adjustability, and even exhibits the LCST-dependent fluorescence behavior, which is a promising stimuli responsive fluorescent polymer for biomedical application.