To further enhance the application of PVB film in the field of antimicrobial technology, the antimicrobial agent curcumin-graphene oxide (Cur-GO) was prepared by adsorption of Cur on the surface of GO using curcumin (Cur) and graphene oxide (GO) as raw materials, and the antimicrobial film Cur-GO/PVB was synthesized by acetalization of polyvinyl butyral (PVB) using polyvinyl alcohol (PVA) and n-butyl aldehyde as raw materials and the antimicrobial film Cur-GO/PVB was prepared by the blending method (PCG). (PCG films). XPS, FTIR, SEM, UV-Vis, TGA, water contact angle, mechanical property, dissolubility tests and bacteriostatic experiments were used to characterize the structure and test the performance of PCG films. The results showed that Cur was successfully adsorbed on the surface of GO, and the UV transmittance of the prepared PCG films in the range of 200~400 nm were all below 29.9%; the onset weight loss temperature (341 ℃) and the maximum decomposition temperature (502 ℃) were both increased than that of the PVB film, and the heat-resistant property was significantly enhanced; the water contact angle was 65.8° at the stable time, and the tensile strength was 17.24 MPa. The elongation at break was 380.7%; the Cur-GO doped PVB films exhibited 90.1% and 89.7% antibacterial activity against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), respectively, through synergistic antimicrobial effects.