A kind of antibacterial silicone rubber modified with silver-loaded silica (SiO2) was prepared by vulcanization, and its antibacterial properties and biological safety were characterized and analyzed by antibacterial plate method, hemolysis and cytotoxicity test. The results show that, when the content of silver-loaded SiO2 antibacterial agent is 20 mg/mL, the antibacterial rate of silicone rubber against Escherichia coli and Staphylococcus aureus reaches 100% and 66%, respectively. Hemolysis test show that, the hemolysis rate of antibacterial silicone rubber is less than 5%. And the cytotoxicity of modified silicone rubber is grade 0. These show that silver-loaded SiO2 modified antibacterial silicone rubber has good antibacterial performance and biological safety. In the field of antibacterial medical catheter, this kind of silicone rubber shows broad clinical application prospect.