Abstract:Based on the characteristics of accurate and controllable sterilization and non-development of bacterial resistance by light, as well as the slightly acidic environment at the site of bacterial infection and the negatively charged, in this paper, two amphiphilic polymers, polyethylene glycol monomethyl ether-b-poly (2 - (diethylamino) ethyl methacrylate) and polyethylene glycol monomethyl ether-b-poly (hydroxyethyl methacrylate-co- diethylamino ethyl methacrylate) (PD and PHD, respectively) were designed and synthesized, which can be protonated in bacterial micro acid. Their micelles were loaded with photosensitizers IR780 and resveratrol (RES) for light sterilization. The structures of the polymers were characterized by NMR and FT-IR. The particle size, size distribution, surface charge, morphology and drug loading of blank and IR780/RES loaded micelles were characterized by dynamic light scattering (DLS), transmission electron microscopy (TEM), ultraviolet (UV) and fluorescence spectroscopy (FL). The results show that PD or PHD micelles and their drug loaded micelles are spherical in structure. The drug loading amounts of IR780 or RES alone in PD or in PHD are very low, while the drug loading amounts of IR780 and RES together in PD or in PHD dramatically increase. The bactericidal rates of the drug loaded micelles against S. aureus and E. coli under 808 nm light are above 99%. The two drug loaded micelles repeatedly demonstrate excellent anti-bactericidal effect.