TiO2-PHMB antimicrobial agent was prepared by grafting poly (hexamethylene biguanide hydrochloride) (PHMB) onto the surface of nano-TiO2 using a silane coupling agent as bridge. Subsequently, the antimicrobial agent was melt-blended with polybutylene adipate terephthalate/polylactic acid (PBAT/PLA) and blown to obtain PBAT/PLA/TiO2-PHMB biodegradable antimicrobial cling film. The study aimed to investigate the effects of different antimicrobial agent contents on the morphological structure, mechanical properties, thermal stability, water vapor permeability, and antimicrobial performance of the films. The results show that when the concentration of TiO2-PHMB antimicrobial agent is 1%, the longitudinal and transverse tensile strength of the PBAT/PLA packaging film are increased by 11.7% and 13.7%, respectively, while maintaining a rupture elongation of 658% and 536%, while the water vapor permeability is decreased by 12.50%. The film inhibitory rates against Escherichia coli (E.coli) and Staphylococcus aureus (S.aureus) reaches 62.70% and 84.54%, respectively, while exhibiting good preservation effects on fresh cherries.